ELISA Inhibition Method
Overview
The ELISA Inhibition Method (EIM) is a competitive ELISA used to quantify anti-dengue IgG antibody titres. Polystyrene plates are coated with human anti-dengue IgG, dengue antigen is added, and test serum is assessed for its ability to inhibit binding of a peroxidase-conjugated anti-dengue IgG conjugate. Read at 492 nm; per cent inhibition is calculated as [1 − (OD sample / OD negative control)] × 100. The antibody titre is the highest serum dilution achieving ≥50% inhibition.
Key Points from Literature
- Used in Garcia2009 - Long-term Clinical Symptoms Post-Dengue to measure anti-dengue IgG titres 2 years after the 2006 Cuban epidemic. Higher titres were significantly associated with persistent clinical symptoms (p = 0.041) and with elevated immune complex levels (p = 0.042).
- Method adapted from Vazquez et al. (2003), originally described for yellow fever vaccination serology.
Contradictions & Debates
None yet recorded.