ELISA
Overview
Enzyme-linked immunosorbent assay (ELISA) is a plate-based immunoassay for detecting and quantifying antibodies, antigens, or proteins in solution. In dengue research, ELISA is used broadly for: (1) measuring specific anti-viral antibody titres (anti-NS1, anti-E IgM/IgG); (2) measuring autoantibody levels against host proteins (anti-PDI, anti-HSP60, anti-vimentin, anti-platelet); and (3) quantifying viral antigens such as NS1 in clinical samples. For dengue-specific serology applications (IgM/IgG classification, NS1 antigen detection), see the specialised pages IgM-IgG Serology ELISA and NS1 Antigen Detection.
Key Points from Literature
Autoantibody quantification in dengue (Cheng2015)
Cheng2015 - NS1 P311-330 Anti-PDI Autoantibodies in DHF used ELISA to quantify anti-PDI, anti-HSP60, anti-vimentin, anti-NS1, and anti-P311–330 IgM and IgG in Vietnamese DHF/DF patient sera. Protocol: 1:25 serum dilution; HRP-conjugated secondary anti-human IgM/IgG; TMB or ABTS substrate; optical density read at 450 nm (TMB) or 405 nm (ABTS). Statistical analysis used linear mixed models (SAS PROC MIXED) to correct for intra-individual correlation in serial serum samples. All autoantibody targets were significantly elevated in DHF vs. normal controls; anti-P311–330 IgM was also significantly elevated in DHF vs. DF, distinguishing disease severity.
Contradictions & Debates
None yet recorded.
Related Pages
- IgM-IgG Serology ELISA
- NS1 Antigen Detection
- Platelet-Associated Immunoglobulin ELISA
- NS1 Molecular Mimicry in Dengue
- Autoimmunity in Dengue
Sources
- Cheng2015 - NS1 P311-330 Anti-PDI Autoantibodies in DHF (anti-PDI, anti-HSP60, anti-vimentin, anti-NS1, anti-P311–330 IgM/IgG by ELISA; 1:25 dilution; HRP-conjugated; TMB/ABTS substrate; linear mixed model analysis; Vietnamese paediatric DHF/DF cohort)